Journal: Journal of Cellular and Molecular Medicine

Article Title: CSF‐1R inhibition disrupts the dialog between leukaemia cells and macrophages and delays leukaemia progression

doi: 10.1111/jcmm.15916

Figure Lengend Snippet: LAMs accumulate in the BM and spleen of T‐ALL mice. A, Schematic illustration of the experimental design. Approximately 2 × 10 6 EL4 cells were transplanted into C57BL/6 mice. When the mice developed T‐ALL at 7 d after EL4 transplantation, CD11b + Ly6G − macrophages were analysed by flow cytometry. B, The ratio of CD11b + Ly6G − macrophages in the BM cells derived from mice transplanted with EL4 cells (right panel), compared to control mice (left panel). C, Ratio of CD11b + Ly6G − macrophages in the spleen derived from mice transplanted with EL4 cells (right panel), compared to control mice (left panel). D, Bar graph shows the absolute numbers of CD11b + Ly6G − macrophages in the BM (left, n = 3) or spleen (right, n = 3) of leukaemic mice, compared to control (out of 10 4 cells acquired by FACS). E, Liver and spleen sections of control (left) or leukaemia mice (right) were stained using an anti‐CD68 antibody to identify macrophages (brown staining). F, Frequency of CD68 + macrophages in the liver (left, n = 5) or spleen (right, n = 5) of control or leukaemia mice

Article Snippet: The antibodies used in present study were FITC anti‐mouse/human CD11B Antibody (Biolegend, Cat. No. 101205, USA), APC anti‐mouse F4/80 Antibody (Biolegend, Cat. No. 123116, USA), APC anti‐mouse CD206 (MMR) Antibody (Biolegend, Cat. No. 141707, USA) and PE anti‐mouse Ly6G Monoclonal Antibody (Elabscience, Cat. No. E‐AB‐F1108D, China).

Techniques: Transplantation Assay, Flow Cytometry, Derivative Assay, Control, Staining

Journal: Journal of Cellular and Molecular Medicine

Article Title: CSF‐1R inhibition disrupts the dialog between leukaemia cells and macrophages and delays leukaemia progression

doi: 10.1111/jcmm.15916

Figure Lengend Snippet: Combination therapy significantly reduces the abundance of LAMs. A, Representative FACS plots showing the ratio of CD11b + Ly6G − macrophages in the BM derived from different treatment groups. B, Representative FACS plots showing the ratio of CD11b + Ly6G − macrophages in the spleen derived from different treatment groups. C, Absolute numbers of CD11b + Ly6G − macrophages in the BM (left) or spleen (right) from different treatment groups (out of 10 4 cells acquired by FACS), (n = 3). D, Liver and spleen sections of different treatment groups were stained using an anti‐CD68 antibody to identify macrophages. E, Frequency of CD68 + macrophages in the liver (left) or spleen (right) of different treatment groups (n = 5). F, Level of IL‐10 in serum of mice from different treatment groups (n = 3)

Article Snippet: The antibodies used in present study were FITC anti‐mouse/human CD11B Antibody (Biolegend, Cat. No. 101205, USA), APC anti‐mouse F4/80 Antibody (Biolegend, Cat. No. 123116, USA), APC anti‐mouse CD206 (MMR) Antibody (Biolegend, Cat. No. 141707, USA) and PE anti‐mouse Ly6G Monoclonal Antibody (Elabscience, Cat. No. E‐AB‐F1108D, China).

Techniques: Derivative Assay, Staining

Journal: PLoS ONE

Article Title: Alarmin Function of Galectin-9 in Murine Respiratory Tularemia

doi: 10.1371/journal.pone.0123573

Figure Lengend Snippet: (A) Frozen sections of lungs harvested at 3 dpi from F.n. infected WT or Gal-9 -/- mice were co-stained with antibodies against myeloid cell markers CD11b (red) and Ly6G (green). A high co-expression of both markers is depicted by yellow color and indicates neutrophils. Nuclei (blue) were stained with 4’6’ diamidino-2-phenylindol-dilactate (DAPI). Magnification X 200. Asterisks depict lesions in the lungs. (B) Flow cytometry analysis of neutrophils in mock control and F.n. infected WT and Gal-9 -/- (WT-Fn and Gal-9 -/- -Fn) mice. Total lungs cells were isolated from mice by collagenase treatment at 3 dpi as described in Methods. The cells were stained with anti-Ly6G-APC and anti-CD11b-Pacific Blue antibodies as markers for neutrophils. The plots are representative of three mice per group in 3 independent experiments. (C) Lungs from mock infected and F.n. infected WT or Gal-9 -/- mice were harvested at 3dpi, homogenized with protease inhibitors in PBS and analyzed commercially for rodent multi-analyte profiles (Myriad Rules-Based Medicine, Austin, TX). Levels of inflammatory cytokines and neutrophil markers in lung homogenates are shown. Results shown are from 3–4 mice per group from 3 different experiments. MMP-9; matrix metalloproteinase 9, MPO; myeloperoxidase. * p <0.05; ** p <0.005; *** p <0.001. Comparisons were made between infected WT and Gal-9 -/- groups.

Article Snippet: For flow cytometry Pacific Blue anti-mouse CD11b and APC anti-mouse Ly6G (Clone 1A8) antibodies (Biolegend, San Diego, CA) were used.

Techniques: Infection, Staining, Expressing, Flow Cytometry, Control, Isolation